ATTCC 100 Test Helps In The Evaluation of antibacterial properties in Fabrics

Biotech engineering and Microbiology have evolved so far that even we can replicate a DNA structure nowadays. It has great participation in the textile and fabric industries. There are many methods to distinguish between good-grade fabrics and bad-grade fabrics. Fabrics need to be an optimal grade before going to the market, and for that, there are quantized methods through which Fabric goes. AATCC 100 test  is one of them by which labs evaluate the antibacterial properties of fabrics. It helps to determine how rigid the Fabric is against bacteria.

Test with AATCC 100

  • Usually, it takes 24 hours to 4 days to complete AATCC 100 test. It through 6 crucial stages, Preparation of samples, sterilization, vaccination, incubation, washing & counting.
  • The microbial culture is incubated & diluted in an aseptic nutritive solution.
  • In accordance with AATCC 100, 1 ml of test organism suspension at about 1000,000 CFU/ml is inoculated to the test sample.
  • Then the control and the test fabric swatches are inoculated with microbes.
  • The vaccination is performed in an aseptic fashion so that the suspension touches only the Fabric.
  • The measurement of bacteria levels of both control and test fabrics are determined at “Time Zero”.
  • An additional control test method is run to verify the perfect neutralization of antimicrobial agents in the sample fabric.
  • In another Sealed jar, another inoculated test and control fabric is allowed to incubate.
  • Decrease in microorganism count in relation to initial concentrations and the control fabric is measured.

Benefits of AATCC 100 Test:

  • This procedure examines both bacteriostatic activity and bactericidal activity of microbial test agents.
  • It helps in the reduction of the growth of detrimental bacteria.
  • The results through AATCC 100  is reproducible. 
  • The results can be a beneficial factor in product marketing at later stages.
  • Microbial concentration is monitored and standardized accordingly using AATCC 100, and the cultured bacteria are provided nutrition during the incubation period. The nutrition helps them to grow against the lacking antibacterial property of the Fabric.
  • And In the other few sample solution, few microbes are putting in a non-nutritive environment. It is also tough to be maintained. 
  • The whole method provides the bacteria with a suitable environment for growth which can provide textile sustainability.

Drawbacks of AATCC 100 Test:

  • Though it has a higher success rate, it also has a minor drawback of suitability on few fabrics, and they oughtn’t to absorb the solution quite properly.
  • If measurements are inaccurate, the result will not be proper.


The U.S.A has made it compulsory to test AATCC 100  test before launching a fabric into the textile market. It provides major freedom to the brand to market their product without any hesitation. With these, tests the textile industries got a parameter to set their standard. And the tests are coming in handy when it comes to stocking the material for a long time.

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